Protectiveeffect of black raspberry seed containing anthocyanins against oxidative damageto DNA, protein, and lipid.
含黑覆盆子种子的花青素对DNA，蛋白质和脂质的氧化损伤的保护作用。
https://link.springer.com/article/10.1007/s13197-015-2094-7
Journal of Food Science andTechnology
February 2016, Volume 53, Issue 2, pp 1214–1221
食品科学技术学报
2016年2月，第53卷，第2期， 第1214-1221页
Authors
Mi-Hee Choi Soon-Mi Shi
作者
Mi-Hee Choi Soon-Mi Shim
This study aimed to determinebioactive components and radical scavenging capacity of black raspberry seedextracts as byproducts obtaining during the juice (FSE) and wine (WSE) makingprocess. Cyanidin-3-O-rutinoside was identified as a major anthocyanin andthe total anthocyanin contents of fresh and wine seed were 78.24 and41.61 mg/100 g of dry weight, respectively. The total phenolic andflavonoid contents of FSE and WSE were 2.31 g gallic acid equivalent (GAE)and 360.95 mg catechin equivalent (CE), and 2.44 g GAE and379.54 mg CE per 100 g dry weight, respectively. The oxygen radicalabsorbance capacity (ORAC) values were 1041.9 μM TE/g for FSE and1060.4 μM TE/g for WSE. Pretreatment of the FSE and WSE inhibited thegeneration of intracellular reactive oxygen species (ROS), DNA and proteindamage induced by hydroxyl radicals, and Fe3+/ascorbic acid-induced lipidperoxidation in a dose dependent manner. WSE more effectively protected fromoxidative damage than FSE. Results from the current study suggest that blackraspberry seeds as byproducts from juice and wine processing could be potentialsources for natural antioxidants.
这项研究旨在确定黑莓树种子提取物的生物活性组分和自由基清除能力，作为果汁（FSE）和葡萄酒（WSE）制备过程中获得的副产物。花青素-3- O芸香糖苷被鉴定为主要的花青素，新鲜和酒籽的总花色苷含量分别为78.24和41.61mg / 100g干重。FSE和WSE的总酚类和类黄酮含量分别为2.31g没食子酸当量（GAE）和360.95mg儿茶素当量（CE），和2.44g GAE和379.54mg CE / 100g干重。氧自由基吸收能力（ORAC）值为FSE为1041.9μMTE/ g和WSE为1060.4μMTE / g。FSE和WSE的预处理抑制了羟自由基诱导的细胞内活性氧（ROS），DNA和蛋白质损伤的产生，并且Fe 3+/抗坏血酸诱导的脂质过氧化作用呈剂量依赖性。WSE比FSE更有效地免受氧化损伤。目前的研究结果表明，作为果汁和葡萄酒加工副产品的黑覆盆子种子可能是天然抗氧化剂的潜在来源。
学术报告2
Black Raspberry Extract Increased Circulating Endothelial ProgenitorCells and Improved Arterial Stiffness in Patients with Metabolic Syndrome: ARandomized Controlled Trial.
黑覆盆子提取物增加循环内皮祖细胞和改善动脉僵硬度在代谢综合征患者：一项随机对照试验。
https://www.ncbi.nlm.nih.gov/pubmed/26891216
J Med Food. 2016 Apr;19(4):346-52. doi:10.1089/jmf.2015.3563. Epub 2016 Feb 18.
J Med食品。2016年4月;19（4）：346-52。doi：10.1089 / jmf.2015.3563。电子书2016年2月18日。
Jeong HS1, Kim S1, Hong SJ1, Choi SC1, Choi JH1, Kim JH1, Park CY1, Cho JY1, Lee TB2, Kwon JW2, Joo HJ1, Park JH1, Yu CW1, Lim DS1.
Authorinformation
Department of Cardiology,Cardiovascular Center, Korea University Anam Hospital , Seoul, Korea.
Gochang Black Raspberry ResearchInstitute , Gochang, Korea.
郑某HS 1，金小号1，香港SJ 1，财SC 1，财JH 1，金JH 1，公园CY 1，卓JY 1，李TB 2，权JW 2，朱HJ 1，公园JH 1，俞CW 1，Lim DS 1。
作者信息
韩国首尔韩国大学Anam医院心血管中心心内科。
高敞黑 覆盆子研究所，韩国高昌。
Raspberries, derived from different cultivar varieties, are apopular ingredient of everyday diet, and their biological activity is a pointof interest for researchers. The ethanol-water extracts from four varieties ofred (Rubus idaeus'Ljulin', 'Veten', 'Poranna Rosa')and black(Rubus occidentalis'Litacz') raspberries were evaluated inthe range of their antimicrobial properties as well as phenolic content -sanguiin H-6, free ellagic acid and anthocyanins. The antimicrobial assay wasperformed with the use of fifteen strains of bacteria, both Gram-negative andGram-positive. The antimicrobial activity of the extracts varied and dependedon the analysed strain of bacteria and cultivar variety, with the exception ofHelicobacter pylori, towards which the extracts displayed the same growthinhibiting activity. Two human pathogens Corynebacterium diphtheriae andMoraxella catarrhalis proved to be the most sensitiveto raspberryextracts. Contrary to the extracts, sanguiin H-6 and ellagicacid were only active against eight and nine bacterial strains, respectively.The determined MIC and MBC values of both compounds were several times lowerthan the tested extracts. The highest sensitivity of Corynebacteriumdiphtheriae to extracts from both black and red raspberries may bedue to its sensitivity to sanguiin H-6 and ellagic acid.
来自不同品种品种的覆盆子是日常饮食的热门成分，它们的生物活性是研究人员感兴趣的一点。来自四种红色（Rubusidaeus'Ljulin'，'Veten'，'Poranna Rosa'）和黑色（悬钩子）的乙醇水提取物occidentalis'Litacz'）覆盆子进行了评估，其抗菌性能以及酚含量 - sanguiin H-6，游离鞣花酸和花青素。使用十五种细菌菌株（革兰氏阴性和革兰氏阳性）进行抗微生物测定。除幽门螺杆菌外，提取物的抗微生物活性各不相同，取决于所分析的细菌菌株和栽培品种，提取物对此表现出相同的生长抑制活性。两种人类病原体白喉棒状杆菌（Corynebacterium diphtheriae）和卡他莫拉菌（Moraxellacatarrhalis）被证明是对覆盆子最敏感的提取物。与提取物相反，sanguiin H-6和鞣花酸仅分别对八种和九种细菌菌株有活性。两种化合物的测定的MIC和MBC值比测试提取物低几倍。白喉棒状杆菌对黑色和红色覆盆子提取物的最高敏感性可能是由于它对三聚氰胺H-6和鞣花酸的敏感性。
原文链接：
https://doi.org/10.1039/c4fo00129j
学术报告3
Effects of Rubus occidentalis extract on blood pressure in patients with prehypertension: Randomized, double-blinded, placebo-controlled clinical trial.
黑覆盆子提取物对高血压前期患者血压的影响：随机，双盲，安慰剂对照临床试验。
https://www.ncbi.nlm.nih.gov/pubmed/26740254
Nutrition. 2016 Apr;32(4):461-7. doi: 10.1016/j.nut.2015.10.014. Epub 2015 Nov 6.
营养。2016年4月; 32（4）：461-7。doi：10.1016 / j.nut.2015.10.014。电子书2015年11月6日。
Jeong HS1, Hong SJ2, Cho JY1, Lee TB3, Kwon JW3, Joo HJ1, Park JH1, Yu CW1, Lim DS1.
Author information
Department of Cardiology, Cardiovascular Center, Korea University Anam Hospital, Seoul, Korea.
Department of Cardiology, Cardiovascular Center, Korea University Anam Hospital, Seoul, Korea. Electronic address: psyche94@gmail.com.
Gochang Black Raspberry Research Institute, Gochang-gun, Korea.
作者信息
韩国首尔韩国大学Anam医院心血管中心心内科。
韩国首尔韩国大学Anam医院心血管中心心内科。电子地址：psyche94@gmail.com。
高敞黑树莓研究所，韩国高敞郡。
Abstract
OBJECTIVE:
Black raspberry (Rubus occidentalis) is known for improving vascular function. However, there has been no study evaluating its effects on 24-h systolic and diastolic blood pressure in prehypertensive patients. The aim of this study was to examine those effects.
目的：
黑覆盆子（Rubus occidentalis）以改善血管功能而闻名。然而，尚未有研究评估其对高血压前期患者24小时收缩压和舒张压的影响。这项研究的目的是检查这些影响。
METHODS:
Patients with prehypertension (N = 45) were prospectively randomized into a moderate-dose black raspberry group (n = 15, 1500 mg/d), a high-dose black raspberry group (n = 15, 2500 mg/d), or a placebo group (n = 15) during an 8-wk follow-up period. Raspberries were consumed in the form of a dried powder extract that was fashioned into capsules. The capsules contained 187.5 and 312.5 mg of raspberry powder, which was equivalent to 1500 and 2500 mg raspberries. Ambulatory 24-h blood pressure (BP); central BP; pulse-wave velocity; abdominal visceral fat; serum renin; angiotensin-converting enzyme; and inflammatory cytokines such as interleukin-6, tumor necrosis factor-α, C-reactive protein, soluble intercellular adhesion molecule-1, soluble vascular cell adhesion molecule-1, and plasminogen activator inhibitor-1 were measured at baseline and at 8-wk follow-up.
方法：
患者高血压前期（N = 45）进行前瞻性随机分为中等剂量黑覆盆子组（n = 15，1500毫克/ d），高剂量黑覆盆子组（n = 15，2500毫克/ d）或安慰剂组（n = 15）在8周的随访期间。树莓被制成胶囊形式的干粉提取物被食用。胶囊含有187.5和312.5毫克覆盆子粉，相当于1500和2500毫克覆盆子。动态24小时血压（BP）; ; 脉搏波速度; 腹部内脏脂肪; 血清肾素; 血管紧张素转换酶; 和炎症因子如白细胞介素-6，肿瘤坏死因子-α，C-反应蛋白，可溶性细胞间粘附分子-1，可溶性血管细胞粘附分子-1，
RESULTS:
High-dose black raspberry significantly reduced 24-h systolic blood pressure (SBP; 3.3 ± 10 mm Hg versus -6.7 ± 11.8 mm Hg; P < 0.05) and nighttime SBP (5.4 ± 10.6 mm Hg versus -4.5 ± 11.3 mm Hg; P < 0.05) compared with controls during the 8-wk follow-up. Black raspberry powder did not produce any significant changes in most of the parameters other than BP.
结果：
高剂量黑覆盆子显着降低24小时收缩压（SBP; 3.3±10mmHg相对于-6.7±11.8mmHg; P <0.05）和夜间SBP（5.4±10.6mmHg相对于-4.5±11.3mmHg;与对照组相比，在8周的随访中P <0.05）。除了BP以外，黑覆盆子粉在大多数参数中没有产生任何显着变化。
CONCLUSION:
The use of black raspberry significantly lowered 24-h BP in prehypertensive patients during the 8-wk follow-up. Black raspberry used as a dietary supplement could be beneficial in reducing SBP in prehypertensive patients.
Copyright © 2016 Elsevier Inc. All rights reserved.
结论：
在8周的随访期间，使用黑覆盆子显着降低了高血压前期患者的24小时血压。用作膳食补充剂的黑覆盆子可能有利于降低高血压前期患者的SBP。
原文：
http://www.nutritionjrnl.com/article/S0899-9007(15)00453-0/fulltext
PMID:26740254 DOI: 10.1016/j.nut.2015.10.014
学术报告4
Characterizationof black raspberry functional food products for cancerprevention human clinical trials.
表征用于癌症预防人类临床试验的黑 覆盆子功能性食品。
https://www.ncbi.nlm.nih.gov/pubmed/24345009
J Agric Food Chem. 2014 May 7;62(18):3997-4006. doi:10.1021/jf404566p. Epub 2013 Dec 27.
J Agric FoodChem。2014年5月7日; 62（18）：3997-4006。doi：10.1021 / jf404566p。电子杂志2013年12月27日。
Gu J1, Ahn-Jarvis JH, Riedl KM, Schwartz SJ, Clinton SK, Vodovotz Y.
Authorinformation
InterdisciplinaryPh.D. Program in Nutrition, ‡Department of Food Science and Technology,§Division of Medical Oncology, Department of Internal Medicine, and#Comprehensive Cancer Center, The Ohio State University , Columbus, Ohio 43210,United States.
谷Ĵ 1，AHN-贾维斯JH，Riedl的KM，施瓦茨SJ，克林顿SK，Vodovotzÿ。
作者信息
跨学科博士学位 美国俄亥俄州立大学俄亥俄州立大学俄亥俄州立大学癌症中心综合肿瘤中心，美国俄亥俄州43210，美国俄亥俄州哥伦布市，食品科学与技术部，医学肿瘤学部，内科。
Our team is designing and fully characterizing black raspberry (BRB)food products suitable for long-term cancer prevention studies. The processing,scale-up, and storage effects on the consistency, quality, bioactive stability,and sensory acceptability of two BRB delivery systems of various matrices arepresented. BRB dosage, pH, water activity, and texture were consistent in thescale-up production. Confections retained >90% of anthocyanins andellagitannin after processing. Nectars had >69% of anthocyanins and >66%of ellagitannin retention, which varied with BRB dosage due to the processingdifference. Texture remained unchanged during storage. BRB products consumed ina prostate cancer clinical trial were well accepted in sensory tests. Thus,this study demonstrates that two different BRB foods can be formulated to meetquality standards with a consistent bioactive pattern and successfully scaledup for a large human clinical trial focusing on cancer risk and other healthoutcomes.
我们的团队正在设计和充分表征黑 覆盆子（BRB）食品适用于长期癌症预防研究。介绍了两种各种基质BRB输送系统的一致性，质量，生物活性稳定性和感官可接受性的处理，放大和储存效果。BRB剂量，pH值，水分活度和质地在放大生产中是一致的。加工后甜食保留> 90％的花青素和鞣花丹宁。花蜜花色苷含量> 69％，鞣花丹素含量> 66％，由于加工差异，花粉含量因BRB剂量而异。储存期间纹理保持不变。在前列腺癌临床试验中消耗的BRB产品在感官测试中被广泛接受。
原文链接：
https://pubs.acs.org/doi/10.1021/jf404566p
学术报告5
Anthocyaninsand their role in cancer prevention.
花青素及其在癌症预防中的作用。
https://www.ncbi.nlm.nih.gov/pubmed/18571839
Cancer Lett. 2008 Oct8;269(2):281-90. doi: 10.1016/j.canlet.2008.05.020. Epub 2008 Jun 20.
癌症通讯。2008年10月8日; 269（2）：281-90。doi：10.1016 /j.canlet.2008.05.020。电子书2008年6月20日。
Wang LS1, Stoner GD.
Authorinformation
Departmentof Internal Medicine and Comprehensive Cancer Center, Ohio State UniversityCollege of Medicine, Columbus, OH 43210, USA.
王LS 1，Stoner GD。
作者信息
俄亥俄州立大学医学院内科及综合癌症中心，美国俄亥俄州43210，哥伦布市。
Anthocyanins are the most abundant flavonoid constituents offruits and vegetables. The conjugated bonds in their structures, which absorblight at about 500 nm, are the basis for the bright red, blue and purple colorsof fruits and vegetables, as well as the autumn foliage of deciduous trees. Thedaily intake of anthocyanins in residents of the United States is estimated tobe about 200 mg or about 9-fold higher than that of other dietary flavonoids.In this review, we summarize the latest developments on the anti-carcinogenicactivities of anthocyanins and anthocyanin-rich extracts in cell culture modelsand in animal model tumor systems, and discuss their molecular mechanisms ofaction. We also suggest reasons for the apparent lack of correlation betweenthe effectiveness of anthocyanins in laboratory model systems and in humans asevidenced by epidemiological studies. Future studies aimed at enhancing theabsorption of anthocyanins and/or their metabolites are likely to be necessaryfor their ultimate use for chemoprevention of human cancer.
花青素是水果和蔬菜中最丰富的类黄酮成分。它们的结构中的共轭键吸收了约500nm的光，是水果和蔬菜的鲜红色，蓝色和紫色以及落叶树的秋天叶子的基础。据估计美国居民花青素的每日摄入量约为200毫克或比其他膳食类黄酮高约9倍。在这篇综述中，我们总结了细胞培养模型和动物模型肿瘤系统中花青素和富含花青素的提取物的抗致癌活性的最新进展，并讨论它们的作用分子机制。我们还建议，由流行病学研究证明，实验室模型系统和人体中花青素的有效性之间缺乏相关性的原因。旨在增强花青素和/或其代谢物吸收的未来研究可能是它们最终用于化学预防人类癌症的必要条件。
原文链接：
https://doi.org/10.1016/j.canlet.2008.05.020
学术报告6
Laboratoryand clinical studies of cancer chemoprevention by antioxidants in berries.
癌症化学预防的抗氧化剂在浆果的实验室和临床研究。
https://www.ncbi.nlm.nih.gov/pubmed/18544560
Carcinogenesis. 2008Sep;29(9):1665-74. doi: 10.1093/carcin/bgn142. Epub 2008 Jun 9.
致癌作用。2008年9月; 29（9）：1665-74。doi：10.1093 / carcin / bgn142。电子书2008年6月9日。
Stoner GD1, Wang LS, Casto BC.
Authorinformation
Departmentof Internal Medicine, College of Medicine, The Ohio StateUniversity, Columbus,OH 43210, USA. gary.stoner@osumc.edu
Stoner GD 1，Wang LS，Casto BC。
作者信息
俄亥俄州立大学医学院内科医学系，美国俄亥俄州43210，哥伦布市。gary.stoner@osumc.edu
premise that diets rich in fruits and vegetables may beprotective against the risk of different types of cancers. Initial evidence forprotective effect of fruits and vegetables against cancer risk came frompopulation-based case-control studies, which prompted intense research aimed at(a) identification of bioactive component(s) responsible for the anticancereffects of fruits and vegetables, (b) elucidation of the mechanisms by whichbioactive food components may prevent cancer, and (c) determination of theirefficacy for prevention of cancer in animal models. The bioactive componentsresponsible for cancer chemopreventive effects of various edible plants havenow been identified. For instance, anticancer effect of Allium vegetablesincluding garlic is attributed to organosulfur compounds (e.g., diallyltrisulfide). Interestingly, unlike cancer chemotherapy drugs, many bioactivefood components selectively target cancer cells. Molecular basis forselectivity of anticancer bioactive food components towards cancer cellsremains elusive, but these agents appear promiscuous and target multiple signaltransduction pathways to inhibit cancer cell growth in vitro and in vivo.Despite convincing observational and experimental evidence, however, limitedeffort has been directed towards clinical investigations to determine efficacyof bioactive food components for prevention of human cancers. This articlereviews current knowledge on cancer chemopreventive effects of a few highlypromising dietary constituents, including garlic-derived organosulfides, berrycompounds, and cruciferous vegetable-derived isothiocyanates, and serves toillustrate complexity of the signal transduction mechanisms in cancerchemoprevention by these promising bioactive food components.
过去三十年的研究提供了令人信服的证据来支持富含水果和蔬菜的饮食可以保护免受不同类型癌症风险的前提。有关水果和蔬菜对抗癌症风险的保护作用的初步证据来自基于人群的病例对照研究，这些研究促进了强烈的研究，旨在（a）鉴定导致水果和蔬菜抗癌作用的生物活性成分，（b ）阐明生物活性食物成分可能预防癌症的机制，以及（c）确定其在动物模型中预防癌症的功效。目前已经确定了负责各种可食用植物的癌症化学预防作用的生物活性成分。例如，包括大蒜的葱属植物的抗癌作用归因于有机硫化合物（例如二烯丙基三硫化物）。有趣的是，与癌症化疗药物不同，许多生物活性食物成分选择性地靶向癌细胞。抗癌生物活性食物组分对癌细胞的选择性的分子基础仍然难以捉摸，但这些药物似乎混杂并且靶向多种信号转导途径以抑制体外和体内癌细胞生长。尽管有令人信服的观察和实验证据，但是，有限的努力已经针对临床研究来确定生物活性食物成分用于预防人类癌症的功效。本文回顾了目前有关几种高度有希望饮食成分的癌症化学预防效果的知识。
原文链接：
https://doi.org/10.1002/jcb.21623
学术报告7
A black raspberry extract inhibits proliferation and regulates apoptosis in cervical cancer cells.
黑覆盆子提取物可抑制宫颈癌细胞增殖并调节细胞凋亡。
https://www.ncbi.nlm.nih.gov/pubmed/21831414
Gynecol Oncol. 2011 Nov;123(2):401-6. doi: 10.1016/j.ygyno.2011.07.023. Epub 2011 Aug 9
Gynecol Oncol。2011年11月; 123（2）：401-6。doi：10.1016 / j.ygyno.2011.07.023。Epub 2011年8月9日。
Zhang Z1, Knobloch TJ, Seamon LG, Stoner GD, Cohn DE, Paskett ED, Fowler JM, Weghorst CM.
Author information
College of Public Health, The Ohio State University, Columbus, OH, USA.
作者信息
俄亥俄州立大学公共卫生学院，美国俄亥俄州哥伦布市。
Abstract
OBJECTIVE:
Cervical cancer is the second most common female cancer worldwide, and it remains a challenge to manage preinvasive and invasive lesions. Food-based cancer prevention entities, such as black raspberries and their derivatives, have demonstrated a marked ability to inhibit preclinical models of epithelial cancer cell growth and tumor formation. Here, we extend the role of black raspberry-mediated chemoprevention to that of cervical carcinogenesis.
目的：
宫颈癌是世界范围内第二大常见女性癌症，对于管理侵入性侵入性病变仍然是一项挑战。基于食物的癌症预防实体，例如黑覆盆子及其衍生物，已经显示出显着抑制上皮癌细胞生长和肿瘤形成的临床前模型的能力。在这里，我们将黑覆盆子介导的化学预防作用扩展到宫颈癌发生的作用。
METHODS:
Three human cervical cancer cell lines, HeLa (HPV16-/HPV18+, adenocarcinoma), SiHa (HPV16+/HPV18-, squamous cell carcinoma) and C-33A (HPV16-/HPV18-, squamous cell carcinoma), were treated with a lyophilized black raspberry ethanol extract (RO-ET) at 25, 50, 100 or 200μg/ml for 1, 3 and 5days, respectively. Cell proliferation was measured by WST1 (tetrazolium salt cleavage) assays. Flow cytometry (propidium iodide and Annexin V staining) and fluorescence microscopy analysis were used to measure apoptotic cell changes.
方法：
将三种人宫颈癌细胞系HELA（HPV16- / HPV18 +，腺癌），SIHA（HPV16 + / HPV18-，鳞状细胞癌）和C-33A（HPV16- / HPV18-，鳞状细胞癌）用冷冻干燥的黑色覆盆子乙醇提取物（RO-ET）分别在25,50,100或200ΜG/ ML下培养1,3和5天。通过WST1（四唑盐切割）测定法测量细胞增殖。使用流式细胞术（碘化丙啶和膜联蛋白V染色）和荧光显微镜分析来测量凋亡细胞变化。
RESULTS:
We found that non-toxic levels of RO-ET significantly inhibited the growth of human cervical cancer cells, in a dose-dependent and time-dependent manner to a maximum of 54%, 52% and 67%, respectively (p<0.05). Furthermore, cell growth inhibition was persistent following short-term withdrawal of RO-ET from the culture medium. Flow cytometry and fluorescence microscopy demonstrated RO-ET-induced apoptosis in all cell lines.
结果：
我们发现RO-ET的无毒水平以剂量依赖性和时间依赖性方式显着抑制人宫颈癌细胞的生长，最大分别为54％，52％和67％（P <0.05） 。此外，细胞生长抑制在培养基中短期停用RO-ET后持续存在。流式细胞术和荧光显微镜显示RO-ET诱导的所有细胞系中的细胞凋亡。
CONCLUSION:
Black raspberries and their bioactive components represent promising candidates for future phytochemical-based mechanistic pathway-targeted cancer prevention strategies.
结论：
黑覆盆子及其生物活性成分代表未来基于植物化学机制途径的癌症预防策略的有希望的候选。
原文：
HTTPS://WWW.NCBI.NLM.NIH.GOV/PMC/ARTICLES/PMC3919135/
PMID:21831414 PMCID:PMC3919135 DOI:10.1016/j.ygyno.2011.07.023
学术报告8
Cancerprevention with freeze-dried berries and berry components.
用冻干浆果和浆果成分预防癌症。
https://www.ncbi.nlm.nih.gov/pubmed/17574861
Stoner GD1, Wang LS, Zikri N, Chen T, Hecht SS, Huang C, Sardo C, Lechner JF.
Authorinformation
Divisionof Hematology and Oncology, Department of Internal Medicine, College ofMedicine, The Ohio State University, Innovation Centre, 2001 Polaris Parkway,Columbus, OH 43240, USA. gary.stoner@osumc.edu
Stoner GD 1，Wang LS，Zikri N，Chen T，Hecht SS，Huang C，Sardo C，Lechner JF。
作者信息
美国俄亥俄州立大学医学院血液与肿瘤学系，创新中心，2001 Polaris Parkway，Columbus，OH 43240，美国。gary.stoner@osumc.edu
Our laboratory is developing a food-based approach to theprevention of esophageal and colon cancer utilizing freeze-dried berries andberry extracts. Dietary freeze-dried berries were shown to inhibit chemicallyinduced cancer of the rodent esophagus by 30-60% and of the colon by up to 80%.The berries are effective at both the initiation and promotion/progressionstages of tumor development. Berries inhibit tumor initiation events byinfluencing carcinogen metabolism, resulting in reduced levels ofcarcinogen-induced DNA damage. They inhibit promotion/progression events byreducing the growth rate of pre-malignant cells, promoting apoptosis, reducingparameters of tissue inflammation and inhibiting angiogenesis. On a molecularlevel, berries modulate the expression of genes involved with proliferation,apoptosis, inflammation and angiogenesis. We have recently initiated clinicaltrials; results from a toxicity study indicated that freeze-dried black raspberries are well tolerated in humans whenadministered orally for 7 days at a dose of 45 g per day. Several Phase IIaclinical trials are underway in patients at high risk for esophagus and coloncancer; i.e., Barrett's esophagus, esophageal dysplasia and colonic polyps, todetermine if berries will modulate various histological and molecularbiomarkers of development of these diseases.
我们的实验室正在开发一种基于食物的方法，以利用冻干浆果和浆果提取物预防食道癌和结肠癌。显示饮食性冻干浆果能够抑制化学诱导的啮齿动物食道癌30-60％，结肠癌高达80％。浆果在肿瘤发生的起始和促进/发展阶段均有效。浆果通过影响致癌物代谢来抑制肿瘤起始事件，导致致癌物诱导的DNA损伤水平降低。它们通过降低恶化前细胞的生长速度，促进细胞凋亡，降低组织炎症参数和抑制血管生成来抑制促进/进展事件。在分子水平上，浆果调节涉及增殖，细胞凋亡，炎症和血管生成的基因的表达。我们最近开始临床试验; 毒性研究结果表明冻干当以45g /天的剂量口服给药7天时，黑覆盆子在人体内耐受性良好。在食管和结肠癌高危患者中正在进行几项IIa期临床试验; 即巴雷特食管，食管发育不良和结肠息肉，以确定浆果是否会调节这些疾病发展的各种组织学和分子生物标志物。
原文链接：
https://www.sciencedirect.com/science/article/pii/S1044579X07000260?via%3Dihub
学术报告9
Molecularmechanisms involved in chemoprevention of black raspberry extracts: from transcription factors totheir target genes.
涉及化学预防黑 覆盆子提取物的分子机制：从转录因子到其靶基因。
https://www.ncbi.nlm.nih.gov/pubmed/16800774
Lu H1, Li J, Zhang D, Stoner GD, Huang C.
Authorinformation
NelsonInstitute of Environmental Medicine, New York University School of Medicine,Tuxedo, NY 10987, USA.
鲁^ h 1，李江，张d，斯托纳GD，黄Ç。
作者信息
纳尔逊环境医学研究所，纽约大学医学院，Tuxedo，纽约10987，美国。
Berries have attracted attention for their chemopreventiveactivities in last a few years. Dietary freeze-dried blackberries have beenshown to reduce esophagus and colon cancer development induced by chemicalcarcinogen in rodents. To elucidate molecular mechanisms involved inchemoprevention by berry extracts, we employed mouse epidermal Cl 41 cell line,a well-characterized in vitro model in tumor promotion studies. Pretreatment ofCl 41 cells with methanol-extracted blackberry fraction RO-ME resulted in adramatical inhibition of B(a)PDE-induced activation of AP-1 and NFkB, andexpression of VEGF and COX-2. The inhibitory effects of RO-ME onB(a)PDE-induced activation of AP-1 and NFkappaB appear to be mediated viainhibition of MAPKs and IkappaBalpha phosphorylation, respectively. In view ofthe important roles of AP-1, NFkappaB, VEGF and COX-2 in tumorpromotion/progression, and VEGF and COX-2 are target of AP-1 and NFkappaB, weanticipate that the ability of black raspberriesto inhibit tumor development may be mediated by impairing signal transductionpathways leading to activation of AP-1 and NFkappaB, subsequently resulting indown-regulation of VEGF and COX-2 expression. The RO-ME fraction appears to bethe major fraction responsible for the inhibitory activity of black raspberries.
在过去的几年里，浆果已经引起了他们化学预防活动的关注。已经表明饮食冷冻干燥的黑莓能够减少啮齿动物中由化学致癌物引起的食管和结肠癌的发展。为了阐明浆果提取物化学预防涉及的分子机制，我们使用了小鼠表皮细胞株，这是一种在肿瘤促进研究中具有良好特征的体外模型。用甲醇提取的黑莓级分RO-ME预处理Cl41细胞导致显着抑制B（a）PDE诱导的AP-1和NFkB活化以及VEGF和COX-2的表达。RO-ME对B（a）PDE诱导的AP-1和NFκB活化的抑制作用似乎分别通过MAPKs和IkappaBalpha磷酸化的抑制来介导。鉴于AP-1，NFkappaB的重要作用，抑制肿瘤发展的黑覆盆子可能通过损伤导致AP-1和NFkappaB激活的信号转导途径来介导，随后导致VEGF和COX-2表达的下调。RO-ME部分似乎是负责黑覆盆子抑制活性的主要部分。
原文链接：
https://www.tandfonline.com/doi/abs/10.1207/s15327914nc5401_8
学术报告10
Pharmacokineticsof anthocyanins and ellagic acid in healthy volunteers fed freeze-dried blackraspberries daily for 7 days.
花青素和鞣花酸在健康志愿者中的药代动力学每天喂食冷冻干燥的黑覆盆子7天。
https://www.ncbi.nlm.nih.gov/pubmed/16172180
J Clin Pharmacol. 2005 Oct;45(10):1153-64.
Stoner GD1, Sardo C, Apseloff G, Mullet D, Wargo W, Pound V, Singh A, Sanders J, Aziz R, Casto B, Sun X.
Authorinformation
Divisionof Hematology/Oncology, Department of Internal Medicine, College of Medicine,The Ohio State University, 1148 James CHRI, 300 W 10th Avenue, Columbus, OH43210, USA.
斯通GD 1，Sardo的Ç，Apseloffģ，鲻鱼d，Wargo w ^，磅V，辛格甲，桑德斯Ĵ，阿兹- [R ，卡斯托乙，太阳X。
作者信息
美国俄亥俄州立大学医学院血液学/肿瘤学系，1148 James CHRI，美国俄亥俄州43210，哥伦布市第10大道300号。
Eleven subjects completed a clinical trial to determine thesafety/tolerability of freeze-dried black raspberries(BRB) and to measure, in plasma and urine, specific anthocyanins-cyanidin-3-glucoside,cyanidin-3-sambubioside,cyanidin-3-rutinoside, and cyanidin-3-xylosylrutinoside, as well as ellagicacid. Subjects were fed 45 g of freeze-dried BRB daily for 7 days. Bloodsamples were collected predose on days 1 and 7 and at 10 time points postdose.Urine was collected for 12 hours predose on days 1 and 7 and at three 4-hourintervals postdose. Maximum concentrations of anthocyanins and ellagic acid inplasma occurred at 1 to 2 hours, and maximum quantities in urine appeared from0 to 4 hours. Overall, less than 1% of these compounds were absorbed andexcreted in urine. None of the pharmacokinetic parameters changed significantlybetween days 1 and 7. In conclusion, 45 g of freeze-dried BRB daily are welltolerated and result in quantifiable anthocyanins and ellagic acid in plasmaand urine.
十一名受试者完成了一项临床试验，以确定冻干黑色的安全性/耐受性覆盆子（BRB），并在血浆和尿液中测量特定的花青素 - 花青素-3-葡萄糖苷，矢车菊素-3-桑布比苷，矢车菊素-3-芸香糖苷和矢车菊素-3-木糖基芸香糖苷以及鞣花酸。受试者每天喂食45g冻干BRB，持续7天。在第1天和第7天以及在给药后10个时间点收集血液样品。在第1天和第7天以及在给药后三个4小时收集尿液12小时。血浆中花青素和鞣花酸的最大浓度发生在1至2小时，尿中最大量出现在0至4小时。总体而言，这些化合物中少于1％被吸收并在尿液中排泄。在第1天和第7天之间，没有任何药代动力学参数发生显着变化。
原文链接：
https://accp1.onlinelibrary.wiley.com/doi/abs/10.1177/0091270005279636