每日摘要:LRR-LRX蛋白的作用于花粉萌发和花粉管生长

2023-05-10 14:56:27


十二月
23

这是郝事之秋陪你的第210天!

Hope everybody can study well and make progress every day!


Pollen-expressed Leucin-rich-repeat extensins are Essential for Pollen Germination and Growth



First author: Xiao-Xiao Wang; Affiliations: Hebei Normal University (河北师范大学): Hebei, China

Corresponding author: Yi Guo (郭毅)


During pollen tube growth, the walls of the tube provide the mechanical strength (机械强度) resisting turgor pressure (膨压) to protect two sperm cells (精细胞). Cell wall proteins may play an important role in this process. Pollen tube cell wall proteins known as leucine-rich repeat (LRR; 富亮氨酸重复序列) extensins (LRXs; 伸展蛋白) harbor an LRR domain and an extensin domain. In this study, the functions of four pollen-expressed LRXs, LRX8, LRX9, LRX10, and LRX11 (LRX8-11), were characterized in Arabidopsis. LRX8-11 displayed a consistent expression pattern in mature pollen grains and pollen tubes. In a phenotypic analysis of four single mutants, six double mutants, four triple mutants, and a quadruple mutant, the triple and quadruple mutant plants displayed markedly reduced seed set (结实) and decreased male transmission efficiency accompanied by compromised (妥协,让步的) pollen germination and pollen tube growth. GFP-fused LRX8, LRX10, and LRX11 were found to be localized to pollen tube cell walls. An immunohistochemical analysis (免疫组织化学分析) of pollen tube cell wall polysaccharides (多糖) showed an increase in the amount of rhamnogalacturonan I (鼠李半乳糖醛酸I) in the sub apical (近尖端的) walls of pollen tubes of the lrx9 lrx10 lrx11 and lrx8 lrx9 lrx11 mutants, and decrease in the content of fucosylated xyloglucans (岩藻糖基化木葡聚糖) in lrx8 lrx9 lrx11 compared to wild-type plants. Moreover, the callose content (胼胝质含量) in the apical walls of pollen tubes increased in the lrx8 lrx9 lrx11 mutant. In conclusion, we propose that LRX8-11 function synergistically (协同地) to maintain pollen tube cell wall integrity; thus, they play critical roles in pollen germination and pollen tube growth.




在花粉管的生长过程中,花粉管壁为保护两个精细胞免受膨压提供机械强度。细胞壁蛋白是一类富亮氨酸重复序列的伸展蛋白LRX,其包括一个LRR结构域和一个伸展蛋白结构域。本文对四个在拟南芥花粉中表达的LRX蛋白LRX8-11进行了研究。LRX8-11在成熟的花粉粒和花粉管中存在一致的表达模式。在4个单突变体、6个双突变体、4个三突变体和1个是突变体的表型分析显示三突和四突植株表现出明显的结实降低,减少了精细胞的传输效率,伴随着花粉萌发和花粉管生长的延缓。GFP融合LRX8,LRX10和LRX11显示定位于花粉管细胞壁中。花粉管细胞壁多糖的免疫组织化学分析显示相对于野生型植株,在lrx9 lrx10 lrx11lrx8 lrx9 lrx11三突变体的花粉管近尖端鼠李半乳糖醛酸I的量有所提升,而在lrx8 lrx9 lrx11三突变体中的岩藻糖基化木葡聚糖含量有所降低。此外,在 lrx8 lrx9 lrx11三突变体的花粉管尖端壁胼胝质含量增加。总的来说,LRX8-11以协同的方式维持花粉管细胞壁的完整性,因此这几个蛋白在花粉萌发和花粉管生长中扮演着重要作用。



通讯:郭毅 (http://school.freekaoyan.com/hebei/hebtu/daoshi/2016/02-15/1455541291494327.shtml)


个人简介:1989.9-1993.6,河北师范大学,理学学士,方向:生物教育;1995.9-1998.6,河北师范大学,硕士,方向:植物生理学;2002.9-2005.6,河北师范大学,博士,方向:细胞生物学;2008.10-2009.10,美国加州大学旧金山分校,高级访问学者。


研究方向:利用蛋白质组学平台,对植物细胞质外体蛋白在植物非生物胁迫逆境反应及发育过程中的功能进行研究。



doi: https://doi.org/10.1104/pp.17.01241


Journal: Plant Physiology

First Published data: 21 December, 2017


(P.S. 欢迎访问个人博客:http://bbs.sciencenet.cn/u/TickingClock


友情链接

Copyright © 2023 All Rights Reserved 版权所有 福建水产设备联盟