讲座主题

Developing Cross-linking Mass Spectrometry to Define Protein Complexes

 讲座时间

2017年3月30日 23:00-23:59

 主讲人简介

Lan Huang, Ph.D., 
Professor Physiology & Biophysics in School of Medicine, 
University of California, Irvine 

Ann Thayer, 
Senior Correspondent, 
C&EN 

 内容简介

In this webinar, we will describe the development of a novel class of MS-cleavable cross-linkers shown to be effective in facilitating the unambiguous identification of cross-linked peptides, as the resulting cross-linked products can be identified based on their characteristic and simplified fragmentation behavior during MS analysis with great utility for in vivo studies. 

The potential of this technology is enormous, and with continued advances in mass spectrometry instrumentation, this new XL-MS strategy has been shown to map in vivo PPIs from mammalian cells at both the proteome scale and the targeted protein complex level. 

Key Learning Objectives

• Learn about why to fully understand protein functions, the proteins involved should be studied in the context of their interactions

• Learn about how cross linking mass spectrometry has emerged as powerful and essential tool for identifying transient protein interactions and refining protein structures

• Learn about the challenges of studying cross linking and the evolution of MS equipment and reagents to meet this need

报名方法

报名请点击“阅读原文”