山东农大团队系统分析拟南芥CYSTM家族非分泌类富含半胱氨酸小肽功能

2023-05-10 14:56:27

近日,Plant and Cell Physiology最新一期杂志发表题为“CYSTM, a Novel Non-Secreted Cysteine-Rich Peptide Family, Involved in Environmental Stresses in Arabidopsis thaliana”的研究论文,系统分析了CYSTM类非分泌富含半胱氨酸的小肽家族的功能。该论文的通信作者为山东农业大学的张世忠和郑成超教授。


Cysteine-rich transmembrane module (CYSTM)是一类小蛋白家族,其特征是尾部具有一个膜锚定结构域。该家族蛋白广泛存在于真核生物中。按照其结构域的保守型分析,这一类蛋白属于一类新的非分泌型富含半胱氨酸的小肽家族(non-secreted cysteine-rich peptides,NCRPs)。目前对于这一类蛋白的作用还知之甚少。本研究中研究人员系统分析了拟南芥中的13个CYSTM基因。CYSTM蛋白可以分为四个主要类别。研究发现各种植物组织中至少有一个CYSTM的成员表达。CYSTM蛋白在植物的多种发育过程尤其是胁迫响应过程中可能具有极其重要的作用。研究发现,CYSTM的蛋白质主要定位于细胞膜和细胞质中。并且其可以通过其末端的结构域形成同源或者异源二聚体。研究人员系统的分析了其相互作用关系并绘制了相互作用图谱。研究还发现,过表达CYSTM3可以负调控盐胁迫下的基因表达。该研究为首次系统分析该类NCRPs的功能。对于进一步分析这一类蛋白在植物中的作用具有重要的启示意义,并提供了坚实的背景基础。

Figure 10. An interaction map in different cellular compartments.

 CYSTM7 and -13 anchored on the plasma membrane could interact with the members located on the plasma membrane or cytoplasm. They might be in a form of dimer or monomer on the membrane without environmental stresses. Lipids were represented with a yellow tail and green head groups. Three possible signalling transduction pathways were proposed: (1) CYSTM7 and -13 might transduce intracellular signal as dimers. (2) CYSTM7 and -13 recognized stress signal molecules, meanwhile, CYSTM4, -6, -11 and -12 might be the intermediate signal molecules of signal transduction. (3) PM-localized CYSTM7 and -13 and cytoplasm-localized CYSTM4, -6, -11 and -12 might coordinate the signaling process.




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